Overexpression of ICAM5 in the Hippocampus of Mice: Construct an adeno-associated virus (AAV) for overexpression of ICAM5. Using stereotactic injection techniques, inject AAV-mCherry or AAV-ICAM5 into the bilateral hippocampal regions of mice, with each mouse receiving 0.8 μL. Three weeks later, randomly select three mice and use immunofluorescence techniques to observe the expression of ICAM5 in the hippocampus and its colocalization with NeuN, MAP2, GFAP, and Iba1.
ICAM5海马过表达AAV构建：立体定位注射与免疫荧光验证方案
本文详细介绍ICAM5海马过表达AAV病毒载体构建方法，涵盖立体定位注射技术参数（双侧海马0.8μL剂量）及三周后免疫荧光验证流程，重点解析ICAM5与NeuN、MAP2、GFAP、Iba1的共定位分析技术要点。
与梅斯小智对话
