线粒体蛋白导入机制：TOM/TIM复合体多方法验证解析

The claim that import occurs through the TOM/TIM machinery is supported by multiple lines of evidence: a conserved N-terminal segment that is necessary and sufficient for mitochondrial targeting (Figure 1F–I), protease protection consistent with matrix residency (Figure 1J–K), and co-immunoprecipitation/mass-spectrometry associations with TOM20/TOM40/TIM23 and HSP60/GRP75 (Figure 1L–O). These results, together with colocalization and fractionation (Figure 1A–E), make a coherent case for canonical presequence import. The breadth of methods is impressive and strongly suggests a role for the presequence pathway.