RNA提取与cDNA合成技术详解

Place 10-20 mg of the sample, stored at -80°C, into an EP tube and add 350 µL of lysis buffer LB. Homogenize the sample using a high-speed low-temperature tissue grinder. Transfer the homogenate to an adsorption column and centrifuge. Discard the filtrate. Repeat the above steps to extract total RNA. Obtain the first-strand cDNA by reverse transcription using the extracted total RNA as the template. Amplify the gene-containing cDNA clone through PCR using the first-strand cDNA as the template.