Place 10-20 mg of the sample, stored at -80°C, into an EP tube and add 350 µL of lysis buffer LB. Homogenize using a high-speed low-temperature tissue grinder. Transfer the homogenate to an adsorption column and centrifuge, discarding the filtrate. Repeat this procedure to extract total RNA. Reverse transcribe the total RNA to obtain the first-strand cDNA. Use this first-strand cDNA as the template for PCR amplification to obtain the gene cDNA clone.
RNA提取与cDNA合成技术指南
本文详细解析RNA提取与cDNA合成的全流程，涵盖样品处理、低温研磨、吸附柱离心等关键步骤，并提供2025年最新实验室技术指南，帮助科研人员高效完成基因克隆实验。
与梅斯小智对话
