UUO模型建立与术后组织采集流程详解

Male C57BL6/J mice aged 6-8 weeks were used to establish the UUO model. After intraperitoneal anesthesia, the mice were fixed and a laparotomy was performed to expose the surgical field using a retractor. The left ureter of the mice was ligated at both the renal and bladder ends and then transected in between. The mice were divided into the UUO group (n=6) and the Sham group (n=6). On postoperative day 7, tissues and organs were harvested from the mice for further study.