生物医学指标检测技术翻译：Western Blot与线粒体功能分析详解

Indicator Testing: Weekly scheduled measurements of body weight and random blood glucose; histopathological examination of gastrocnemius muscle tissue (H&E staining, PAS staining); transmission electron microscopy observation of mitochondrial ultrastructure; enzymatic activity detection using kits for L-LDH and G6PDH; Western Blot analysis of GLUT4, MSTN, AMPK, P-AMPK, PGC-1α, NRF2, TFAM, OPA1, MFN2, DRP1, PINK1, and PARKIN protein expression; real-time quantitative PCR detection of mitochondrial DNA copy number.