H&E染色技术解析：皮肤组织病理变化的时间序列观察

H&E staining showed that at the four examination time points (D1 to D14), the cells in the normal control group were arranged neatly and tightly, with a complete and clear epidermal structure. The collagen fibers in the dermis were densely and intricately arranged, the cytoplasm was uniformly eosinophilic, and the structures of appendages such as hair follicles and sebaceous glands were intact. The subcutaneous fat layer and muscle layer were clearly visible, with adipocytes and muscle fibers arranged neatly and densely, uniform in size, and with normal nuclear morphology. In the model control group, the epidermis exhibited incomplete keratinization, necrosis of varying degrees, and loss of structure. Cells were disintegrated and fragmented, with thickening observed. The collagen fibers in the dermis also showed necrosis of varying degrees and loss of structure, with pale cytoplasmic staining and pyknotic or absent nuclei. Necrotic areas were accompanied by inflammatory cell infiltration, primarily lymphocytes and neutrophils. Fibrous tissue showed varying degrees of hyperplasia, and there was mild hemorrhage in some areas with a few red blood cells accumulating. The conditions were particularly severe on D1 and D3, while the inflammatory cell infiltration was somewhat reduced on D7 and D14.