- MICT and HIIT Downregulate MSTN and Activate the AMPK/PGC-1α Signaling Axis
Compared to the NC group, the protein expression of PGC-1α, AMPK, and P-AMPK in the DC group was significantly decreased (P<0.001, P<0.01), while the protein expression of MSTN was significantly increased (P<0.01). Compared to the DC group, the protein expression of PGC-1α and P-AMPK in both the MICT and HIIT groups was significantly increased (P<0.01, P<0.001), and the protein expression of MSTN was significantly decreased (P<0.001). The protein expression of AMPK showed no significant change in the MICT group but was significantly increased in the HIIT group (P<0.001). Compared to the MICT group, the protein expression of PGC-1α, AMPK, and P-AMPK in the HIIT group was significantly increased (P<0.01, P<0.05), while the protein expression of MSTN showed no significant change.
Research Conclusions:
- Both HIIT and MICT interventions can improve body weight, blood glucose levels, and skeletal muscle glucose metabolism in T2DM mice, enhancing the quality of skeletal muscle mitochondria, with HIIT showing superior effects compared to MICT.
- Both HIIT and MICT interventions downregulate the expression of MSTN in skeletal muscle, activate the AMPK/PGC-1α signaling axis, and promote mitochondrial quality control, thereby improving glucose metabolic disorders in the skeletal muscle of T2DM mice.
