雷公藤甲素如何保护Huh-7肝癌细胞？2024线粒体功能机制解析

Routine in vitro cultivation of the Huh-7 human hepatocellular carcinoma cell line was performed, and cells were treated with a gradient concentration (0, 0.25, 0.5, 1, 2, 4 μM) of triptolide. The CCK-8 cell viability assay was used to determine the highest drug concentration that does not affect cell viability. The experiment was divided into four groups: control group, H2O2 group, H2O2 + DMSO group, and H2O2 + triptolide group. The degree of cellular senescence was assessed using β-galactosidase staining. Intracellular reactive oxygen species (ROS) levels were detected using the DCFH-DA fluorescence probe method. Intracellular ATP levels were measured using the luciferase catalysis method. Superoxide levels in the mitochondrial matrix were detected using the MitoSO Red fluorescence probe method. Mitochondrial membrane potential was assessed using the JC-1 fluorescence probe method.