The mouse kidneys were homogenized in Trizol, and the target gene transcription levels were quantitatively analyzed through two steps: reverse transcription and real-time quantitative PCR. The relative expression values of the target genes in the samples were calculated using the 2-ΔΔCT method, and the fold change was normalized to the average expression level of the Sham group. The primers used in this study are as follows:
- Mouse Gapdh forward: 5’-AGTGTTTCCTCGTCCCGTAG-3’
- Mouse Gapdh reverse: 5’-GCCGTGAGTGGAGTCATACT-3’
- Mouse Fn1 forward: 5’-CCCTATCTCTGATACCGTTGTCC-3’
- Mouse Fn1 reverse: 5’-TGCCGCAACTACTGTGATTCGG-3’
- Mouse Col1a1 forward: 5’-CCTCAGGGTATTGCTGGACAAC-3’
- Mouse Col1a1 reverse: 5’-CAGAAGGACCTTGTTTGCCAGG-3’
- Mouse Ccl2 forward: 5’-GCTACAAGAGGATCACCAGCAG-3’
- Mouse Ccl2 reverse: 5’-GTCTGGACCCATTCCTTCTTGG-3’
