rMAP治疗对病变组织病理学变化的影响分析

3. Experimental Results 3.1 Analysis of Histopathological Changes in Lesional Tissues at Different Time Points Following rMAP Treatment HE staining showed that at the four examination time points (D1 to D14), the cells in the normal control group were arranged neatly and tightly, with a complete and clear structure of the epidermis, dense and interwoven collagen fibers in the dermis, uniformly eosinophilic cytoplasm, intact structures of appendages such as hair follicles and sebaceous glands, and clearly visible subcutaneous fat layer and muscle layer. The adipocytes and muscle fibers were arranged neatly and densely, with uniform size and normal nuclear morphology. In the model control group, the epidermis exhibited incomplete keratinization and varying degrees of necrosis, with structural loss, cell disintegration, fragmentation, and thickening. The collagen fibers in the dermis showed varying degrees of necrosis and structural loss, with pale-stained cytoplasm, pyknotic or absent nuclei, and inflammatory cell infiltration, primarily consisting of lymphocytes and neutrophils. There was also varying degrees of fibrous tissue hyperplasia and mild hemorrhage with the accumulation of a few red blood cells, particularly severe at D1 and D3. By D7 and D14, the inflammatory cell infiltration had somewhat decreased compared to D1 and D3. In the rMAP group and the collagen group at D1 and D3, the epidermis exhibited incomplete keratinization and necrosis, with some areas showing the formation of new epidermis. The dermis contained a large number of necrotic collagen fibers accompanied by inflammatory cell infiltration, primarily consisting of lymphocytes and neutrophils. Compared to the model group, the rMAP group and the collagen group showed gradual improvement in the repair of the epidermis and dermis and a reduction in inflammatory cell infiltration at D3, D7, and D14. See Figure 1.