重组贻贝粘附蛋白在皮肤损伤修复中的应用研究

The skin is the largest organ on the body surface, and various physical and chemical factors can cause skin damage. Common types of injuries include trauma, scalds, and burns. The healing process of these wounds generally involves four stages: hemostasis, inflammation, proliferation, and remodeling. The inflammatory response phase is a crucial stage in regulating wound repair, with multiple inflammatory cells and cytokines working together to combat infection [1]. Recombinant mussel adhesive protein (rMAP) is a large molecular protein obtained through genetic engineering techniques using microorganisms as host bacteria, followed by fermentation and purification processes. The rMAP protein sequence is rich in dopamine, lysine, and arginine, which allows the protein to adhere tightly and stably to the skin surface, providing protection and promoting wound healing. rMAP is widely used clinically for the care of acute wounds (such as those after laser/photonic/chemical peeling, dermatitis, eczema, sensitive skin, and acne) and surrounding skin. Studies have shown that rMAP plays a positive role in the hemostasis and inflammation phases of wound healing, inhibiting inflammatory cytokines such as IFN-γ, IL-2, IL-6, and TNF-α in the skin wound tissue. To further investigate the effects and mechanisms of rMAP on skin wound repair and promotion of healing, the author simulated acute skin wounds in rats using laser irradiation and explored the impact of rMAP on skin wound repair, the inflammatory process, and the levels of inflammatory cytokines. Additionally, to further study the mechanisms by which rMAP promotes cell repair in skin wounds, the author measured the levels of PI3K, AKT, mTOR, and their phosphorylation in skin wound tissues using Western blotting (WB), and detected the mRNA levels of EGF, FGF-2, and VEGF using PCR. These findings aim to elucidate the mechanisms by which rMAP promotes wound healing, providing a therapeutic basis for the clinical application of rMAP in wound repair following laser and other aesthetic medical procedures.