rMAP促进伤口愈合的分子机制研究

Real-time PCR was used to detect the expression levels of EGF, FGF-2, and VEGF genes. The results showed that compared to the normal group, the mRNA expression levels of EGF, FGF-2, and VEGF in the skin tissue of rats in the model group were significantly reduced (P < 0.01). The EGF mRNA expression level decreased from 1.051 ± 0.342 to 0.157 ± 0.067, the FGF-2 mRNA expression level decreased from 1.005 ± 0.123 to 0.521 ± 0.127, and the VEGF mRNA expression level decreased from 1.019 ± 0.231 to 0.226 ± 0.031. Compared to the model control group, the mRNA expression levels of EGF in the skin tissue of rats treated with rMAP and collagen protein were significantly increased (P < 0.05), rising from 0.157 ± 0.067 to 0.412 ± 0.182. The mRNA expression levels of FGF-2 and VEGF in the skin tissue of rats treated with rMAP were also significantly increased (P < 0.05), with FGF-2 mRNA expression increasing from 0.521 ± 0.127 to 0.879 ± 0.179 and VEGF mRNA expression increasing from 0.226 ± 0.031 to 0.435 ± 0.102. In summary, rMAP can promote wound healing by increasing the expression levels of VEGF, FGF-2, and EGF mRNA in granulation tissue, exerting the combined regulatory effects of these factors, promoting the proliferation of fibroblasts and new capillaries, improving blood circulation at the wound site, promoting granulation tissue growth and filling, accelerating epithelial cell growth, and facilitating wound healing.