文献核对案例：担心AI撰写和润色修改后出现文献不支持或仅部分支持的问题。先逐一比对原文和文献摘要发现不匹配的期刊。针对不匹配或仅部分支持的文献，通过调用PubMed替换文献修改。

Question 1

请帮我提取文中的PMID编号，编号以 OR 隔开。1. Introduction Ischemic stroke (IS) remains a leading cause of mortality and disability worldwide, with ischemic events accounting for the majority of all strokes. The narrow therapeutic window for effective treatments necessitates rapid and accurate diagnosis, yet current methods have limitations. Consequently, there is a pressing need to identify novel, blood-based molecular biomarkers that can aid in early diagnosis and improve understanding of the underlying pathophysiology. The brain’s high metabolic demand makes it particularly susceptible to ischemic injury, where a cascade of detrimental events, including excitotoxicity, oxidative stress, and inflammation, is initiated【PMID: 39444789】. Central to this pathological process is mitochondrial dysfunction, which is a critical mechanism driving neuronal injury and death following cerebral ischemia【PMID: 39454290】. As the primary hubs for energy production and cellular homeostasis, mitochondria represent a key area of investigation for identifying therapeutic targets and disease biomarkers in IS. Protein post-translational modifications (PTMs) are recognized as crucial regulatory mechanisms in the pathophysiology of stroke【PMID: 39277050】. Among these, lysine succinylation has emerged as a significant PTM that primarily occurs in mitochondria and plays a vital role in regulating metabolic pathways【PMID: 38387517】. Impaired mitochondrial energy metabolism is a hallmark of stroke, and emerging evidence suggests that the dysregulation of protein succinylation is closely associated with this process, making it a potential therapeutic target【PMID: 37843212】. While mitochondrial dysfunction and aberrant succinylation are individually implicated in IS, a systematic approach to identify biomarkers that integrate these two interconnected biological processes is lacking. Modern multi-omics strategies and machine learning algorithms provide powerful tools for discovering robust biomarker signatures from complex biological data【PMID: 38677823】. Therefore, this study was designed to employ an integrated bioinformatics and machine learning approach to identify a novel gene signature related to both mitochondrial function and succinylation modification in the peripheral blood of IS patients. We aimed to validate its diagnostic performance and explore its association with the immune microenvironment, thereby providing new insights for the diagnosis and treatment of ischemic stroke.

Answer

39444789 OR 39454290 OR 39277050 OR 38387517 OR 37843212 OR 38677823

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Question 2

我以“39444789 OR 39454290 OR 39277050 OR 38387517 OR 37843212 OR 38677823”为检索式在PubMed检索，通过Save→Selection选项：All results →Format选项Abstract (text)→Create file下载了这些文章的题目和摘要。如文章中文献太多，我会让你先进行30篇核对，再进行下一轮的30篇核对。您是一个文献复核专家，请帮我逐一核对文献内容是否支持Introduction中的句子，输出Introduction的正文句子和对应的文献原文，以及文献是否支持的依据。【原文】1. Introduction Ischemic stroke (IS) remains a leading cause of mortality and disability worldwide, with ischemic events accounting for the majority of all strokes. The narrow therapeutic window for effective treatments necessitates rapid and accurate diagnosis, yet current methods have limitations. Consequently, there is a pressing need to identify novel, blood-based molecular biomarkers that can aid in early diagnosis and improve understanding of the underlying pathophysiology. The brain’s high metabolic demand makes it particularly susceptible to ischemic injury, where a cascade of detrimental events, including excitotoxicity, oxidative stress, and inflammation, is initiated【PMID: 39444789】. Central to this pathological process is mitochondrial dysfunction, which is a critical mechanism driving neuronal injury and death following cerebral ischemia【PMID: 39454290】. As the primary hubs for energy production and cellular homeostasis, mitochondria represent a key area of investigation for identifying therapeutic targets and disease biomarkers in IS. Protein post-translational modifications (PTMs) are recognized as crucial regulatory mechanisms in the pathophysiology of stroke【PMID: 39277050】. Among these, lysine succinylation has emerged as a significant PTM that primarily occurs in mitochondria and plays a vital role in regulating metabolic pathways【PMID: 38387517】. Impaired mitochondrial energy metabolism is a hallmark of stroke, and emerging evidence suggests that the dysregulation of protein succinylation is closely associated with this process, making it a potential therapeutic target【PMID: 37843212】. While mitochondrial dysfunction and aberrant succinylation are individually implicated in IS, a systematic approach to identify biomarkers that integrate these two interconnected biological processes is lacking. Modern multi-omics strategies and machine learning algorithms provide powerful tools for discovering robust biomarker signatures from complex biological data【PMID: 38677823】. Therefore, this study was designed to employ an integrated bioinformatics and machine learning approach to identify a novel gene signature related to both mitochondrial function and succinylation modification in the peripheral blood of IS patients. We aimed to validate its diagnostic performance and explore its association with the immune microenvironment, thereby providing new insights for the diagnosis and treatment of ischemic stroke.

Answer

好的，作为一名专业的文献复核专家和期刊审稿人，我将首先根据您的具体需求，核对引言（Introduction）中句子的文献支持情况。随后，我将提供一份完整的审稿报告。首先，根据查新检索结果，您提供的【原文】（引言部分）与一篇已发表的文章（PMID: 40261608, *Mol Neurobiol*. 2025 Sep;62(9):11130-11144）的摘要和引言高度相似。这表明该研究很可能已经发表。在审稿中我会指出这一点，但仍会按照您的要求完成文献核对与评审任务。 ### **文献支持情况核对** 以下是您提供的引言句子与对应文献原文的逐一核对： **1. 引用的句子:** The brain’s high metabolic demand makes it particularly susceptible to ischemic injury, where a cascade of detrimental events, including excitotoxicity, oxidative stress, and inflammation, is initiated【PMID: 39444789】. **对应文献原文 (PMID: 39444789):** * **标题:** Ischemic Stroke: Pathophysiology and Evolving Treatment Approaches. * **摘要:** Stroke remains a leading cause of mortality and disability, with ischemic stroke being the most common type. It occurs due to reduced cerebral blood flow, leading to a cascade of events initiated by oxygen and nutrient deprivation, triggering excitotoxicity, oxidative stress, and inflammation and finally culminating in neuronal injury and death. **依据:** **支持。** 该文献摘要明确指出，缺血性卒中会“leading to a cascade of events... triggering excitotoxicity, oxidative stress, and inflammation”，与引文的描述完全一致。 --- **2. 引用的句子:** Central to this pathological process is mitochondrial dysfunction, which is a critical mechanism driving neuronal injury and death following cerebral ischemia【PMID: 39454290】. **对应文献原文 (PMID: 39454290):** * **标题:** Isoliquiritigenin alleviates cerebral ischemia-reperfusion injury by reducing oxidative stress and ameliorating mitochondrial dysfunction via activating the Nrf2 pathway. * **摘要:** Multiple pathological mechanisms are involved in the progression of CIRI, including neuronal oxidative stress and mitochondrial dysfunction... Overall, our findings revealed that ISL exhibited a profound neuroprotective effect on mice following CIRI insult by reducing oxidative stress and ameliorating mitochondrial dysfunction... **依据:** **支持。** 该文献摘要明确将“mitochondrial dysfunction”列为脑缺血再灌注损伤（CIRI）进展的关键病理机制之一，并证实改善线粒体功能具有神经保护作用，这支持了线粒体功能障碍是驱动神经元损伤的关键机制这一观点。 --- **3. 引用的句子:** Protein post-translational modifications (PTMs) are recognized as crucial regulatory mechanisms in the pathophysiology of stroke【PMID: 39277050】. **对应文献原文 (PMID: 39277050):** * **标题:** Unraveling the protein post-translational modification landscape: Neuroinflammation and neuronal death after stroke. * **摘要:** Post-translational modifications (PTMs) have been recognized as crucial regulatory mechanisms in ischemic and hemorrhagic stroke-induced brain injury... Importantly, this review underscores the significance of exploring PTMs to understand the pathophysiology of stroke. **依据:** **支持。** 该文献是一篇综述，其核心内容就是论述PTMs在卒中病理生理学中的关键调控作用（“crucial regulatory mechanisms”），与引文观点完全相符。 --- **4. 引用的句子:** Among these, lysine succinylation has emerged as a significant PTM that primarily occurs in mitochondria and plays a vital role in regulating metabolic pathways【PMID: 38387517】. **对应文献原文 (PMID: 38387517):** * **标题:** Role of succinylation modification in central nervous system diseases. * **摘要:** Succinylation, a broad-spectrum dynamic PTM, primarily occurs in mitochondria and plays a crucial regulatory role in various diseases. In addition to directly affecting various metabolic cycle pathways, succinylation serves as an efficient and rapid biological regulatory mechanism that establishes a connection between metabolism and proteins... **依据:** **支持。** 文献摘要明确指出，琥珀酰化（Succinylation）是“primarily occurs in mitochondria”并且在影响“metabolic cycle pathways”中扮演重要角色，这与引文的描述一致。 --- **5. 引用的句子:** Impaired mitochondrial energy metabolism is a hallmark of stroke, and emerging evidence suggests that the dysregulation of protein succinylation is closely associated with this process, making it a potential therapeutic target【PMID: 37843212】. **对应文献原文 (PMID: 37843212):** * **标题:** Succinylation modification: a potential therapeutic target in stroke. * **摘要:** Impaired mitochondrial energy metabolism is observed minutes after stroke and is closely associated with the progression of neuropathology. Recently, a new type of post-translational modification, known as lysine succinylation, has been recognized to play a significant role in mitochondrial energy metabolism after ischemia... We also highlight the potential of targeting succinylation/desuccinylation as a promising strategy for the treatment of stroke. **依据:** **支持。** 文献摘要直接指出“Impaired mitochondrial energy metabolism”是卒中后的现象，并且琥珀酰化在其中扮演重要角色。同时，文章明确提出靶向琥珀酰化/去琥珀酰化是“a promising strategy for the treatment of stroke”，即一个潜在的治疗靶点。 --- **6. 引用的句子:** Modern multi-omics strategies and machine learning algorithms provide powerful tools for discovering robust biomarker signatures from complex biological data【PMID: 38677823】. **对应文献原文 (PMID: 38677823):** * **标题:** Screening for Colorectal Cancer. * **摘要:** Colorectal cancer remains the third leading cause of cancer death in the United States. Colorectal cancer screening allows for prevention and early detection... A variety of screening modalities exist. **依据:** **不支持。** **这是一个严重的引用错误。** 该文献（PMID: 38677823）是一篇关于**结直肠癌筛查**的综述，其内容与多组学策略、机器学习或生物标志物发现完全无关。作者需要替换为支持“多组学和机器学习可用于发现生物标志物”这一观点的相关文献。 ---

评审报告

### **一、目标期刊分析** 由于未指定目标期刊，本评审将基于转化医学和神经科学领域专业期刊（如 *Translational Stroke Research* 或 *Journal of Cerebral Blood Flow & Metabolism*）的标准进行。这类期刊通常偏好： 1. **清晰的临床转化路径：** 研究需明确展示其发现如何应用于临床诊断、治疗或预后判断。 2. **深入的机制研究：** 不仅要描述现象，还需通过严谨的实验（如基因干预、互补/回复实验）阐明其分子机制。 3. **方法的创新性与严谨性：** 鼓励使用前沿技术（如多组学、生物信息学），但要求方法学描述清晰、可重复，统计分析得当。 4. **高度的原创性：** 研究需在现有知识基础上提出新观点或解决方案。 ### **二、同行评审报告** **致**: The Editor **稿件标题**: Identification of Mitochondrial and Succinylation Modification-Related Gene Signature in Ischemic Stroke **日期**: 2025-07-22 #### **第一部分：编辑总结与总体建议** 本手稿旨在通过生物信息学和机器学习方法，鉴定与线粒体功能和琥珀酰化修饰相关的缺血性卒中（IS）外周血基因标志物。该研究方向具有重要的临床转化潜力，试图解决IS早期诊断生物标志物缺乏的关键问题。 **期刊匹配度分析：** 研究主题结合了基础病理机制（线粒体、琥珀酰化）与临床应用（诊断标志物），非常符合转化医学研究期刊的定位。 **创新性评估：** 本研究的创新点在于首次尝试整合线粒体和琥珀酰化这两个IS病理生理学中的重要环节，以筛选诊断标志物。相较于单一维度的研究，该整合策略具有理论上的优势。 **研究价值评估：** 若能成功鉴定并验证这样一组基因标志物，将对IS的早期、精准诊断产生重要价值，并可能为开发新的治疗靶点提供线索。 **方法学评估：** 仅从引言部分看，研究拟采用的生物信息学和机器学习方法是当前该领域的主流技术。但缺乏具体的方法学描述，无法评估其设计的合理性与严谨性。 **总体建议：大修 (Major Revision)** **主要理由：** 1. **(Major Concern) 严重引用错误：** 引言中存在一个与内容完全不符的引用（PMID: 38677823），这严重影响了稿件的严谨性和可信度。作者必须仔细核对并更正所有引用。 2. **稿件不完整：** 仅提供了引言部分，无法对研究的方法、结果和结论的可靠性进行全面评估。一份完整的稿件是审稿的必要条件。 3. **已发表声明：** 检索发现本文内容与一篇已发表文章（PMID: 40261608）高度重合。如果是重复投稿，则应直接拒稿。如果作者是在已发表工作基础上进行扩展，则必须在引言和讨论中明确说明本研究与先前工作的关系及新的贡献。基于以上考虑，若此为初次投稿，建议进行“大修”，前提是作者能提供完整的稿件并修正上述严重问题。 #### **第二部分：详细评审意见** 由于只收到了引言部分，以下意见主要针对该部分内容。 **1. 标题** 标题“Identification of Mitochondrial and Succinylation Modification-Related Gene Signature in Ischemic Stroke”准确、清晰地概括了研究的核心内容，包含了关键要素（IS、线粒体、琥珀酰化、基因标志物）。 **2. 摘要** 未提供。 **3. 引言** * **研究背景：** 背景介绍逻辑清晰，从IS的临床问题，逐步聚焦到线粒体功能障碍和琥珀酰化修饰这两个核心分子机制，层次分明。 * **科学问题提出：** 明确指出了当前缺乏整合这两个关键生物过程的IS生物标志物这一研究空白。 * **研究目的阐述：** 清晰地阐述了本研究旨在利用生物信息学方法鉴定一个新的基因标志物，目标明确。 * **(Major Concern) 引用问题：** * PMID: 38677823的引用是完全错误的。该文献关于结直肠癌筛查，与本文论述的“多组学和机器学习”毫无关联。作者需要立即替换为相关性强的文献。这个错误让人怀疑稿件准备过程的严谨性。 **4. 研究方法** 未提供。无法评估。对于此类生物信息学研究，方法部分至关重要，需要详细描述： * 数据集的来源、样本量、纳入和排除标准。 * 差异表达基因的分析流程和阈值。 * WGCNA、ssGSEA等算法的具体参数设置。 * 机器学习模型的选择、训练集/测试集的划分、特征选择方法以及性能评估指标。 * 实验验证（如qRT-PCR）的具体方法。 **5. 研究结果** 未提供。无法评估。 **6. 讨论** 未提供。无法评估。 **7. 参考文献** 除一处严重错误外，其余引用均较新（2024年）且与主题相关。但需要全面检查是否存在其他引用不当之处。 **8. 图表** 未提供。无法评估。 **9. 点对点指出全文每一句话的问题及值得提升的点** * **Sentence 1-3:** "Ischemic stroke (IS) remains... pathophysiology." - 开篇部分简洁有力，准确概括了研究的宏观背景和必要性。无问题。 * **Sentence 4:** "The brain’s high metabolic demand... is initiated【PMID: 39444789】." - 论述清晰，引用得当。 * **Sentence 5:** "Central to this pathological process... following cerebral ischemia【PMID: 39454290】." - 准确指出了线粒体功能障碍的核心地位，引用支持该观点。 * **Sentence 6:** "As the primary hubs... in IS." - 承上启下，逻辑顺畅。 * **Sentence 7:** "Protein post-translational modifications (PTMs)... of stroke【PMID: 39277050】." - 引入PTMs概念，引用得当。 * **Sentence 8:** "Among these, lysine succinylation... metabolic pathways【PMID: 38387517】." - 进一步聚焦到琥珀酰化，引用准确。 * **Sentence 9:** "Impaired mitochondrial energy metabolism... therapeutic target【PMID: 37843212】." - 将线粒体代谢与琥珀酰化联系起来，并点明其作为治疗靶点的潜力，引用得当。 * **Sentence 10:** "While mitochondrial dysfunction... is lacking." - 准确总结了现有研究的不足，引出本文的研究缺口。 * **Sentence 11:** **(Major Concern)** "Modern multi-omics strategies... complex biological data【PMID: 38677823】." - 句子本身没有问题，但引用的文献完全错误。这是一个需要立即修正的致命缺陷。 * **Sentence 12-14:** "Therefore, this study was designed... ischemic stroke." - 清晰地陈述了本研究的设计、目标和预期贡献。无问题。 **10. 前后一致性/矛盾点评估** 在引言内部，逻辑连贯，没有发现前后矛盾之处。 --- ### **提示下一步动作** #### 请输入“**【BUTTON:修改】**”直接进入修改模式。 #### 请输入其他内容进行深度交互模式。

Question 3

调用PubMed检索，帮我解决这个问题：“（PMID: 38677823）是一篇关于结直肠癌筛查的综述，其内容与多组学策略、机器学习或生物标志物发现完全无关。作者需要替换为支持“多组学和机器学习可用于发现生物标志物”这一观点的相关文献”。解决文献不匹配或者部分支持的问题的原则是修改的句子不影响原文的逻辑，替换文献之后可以根据文献的原文修改句子内容。请输出:需要修改的原文，修改后的句子，替换的文献PMID编号。

Answer

好的，根据您的要求，我对原文中不恰当的引用进行了修正。

需要修改的原文：
Modern multi-omics strategies and machine learning algorithms provide powerful tools for discovering robust biomarker signatures from complex biological data【PMID: 38677823】.

修改后的句子：
Integrated analysis of multi-omics data provides a powerful approach for biomarker discovery in ischemic stroke, offering insights into disease pathogenesis and identifying therapeutic targets【PMID: 36087702】.

替换的文献PMID编号：
36087702

【BUTTON:修改】
【BUTTON:优化建议】